The first method is a reduced representation sequencing which maximizes capture of RNAs and preserves transcripts’ directionality. The second, a template-switch protocol, is designed for small mammalian neurons. Both protocols, from cell/tissue isolation to final sequence data, take up to 4 days. The efficiency of these protocols has been validated with single hippocampal neurons and various invertebrate tissues including individually identified neurons within a simpler memory-forming circuit of Aplysia californica and early (1-, 2-, 4-, 8-cells) embryonic and developmental stages from basal metazoans.ĭNA and RNA sequencing has and will continue to make a profound impact on medicine, clinical, and basic research. It has become indispensable for virtually any biomedical field, providing an unbiased view of the entire molecular machinery within a biological system of interest. The current trends are to adapt high-throughput sequencing technologies to the level of small-cell populations and even individual cells.
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